LOCUS       40924_23694       12183 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB709 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12183)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12183)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12183)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1195
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     protein_bind    1205..1329
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1366..1396
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1450..2106
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2451..2753
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2797..2921)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2947..3199
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3233..3249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3257..3273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3281..3311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3326..3347)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3383..3635)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3673..4968)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61184.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3673..4968)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5020..5199)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5705..5729)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6250..7038
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7287..7875
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8061..8201)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8545..8739)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8808..9878)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10310..10936)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagccat catcatcatc atcatgctgt
     1201 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     1261 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     1321 gtcactatgg cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct
     1381 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     1441 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     1501 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     1561 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     1621 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     1681 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     1741 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     1801 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     1861 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     1921 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     1981 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     2041 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2101 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     2161 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     2221 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     2281 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     2341 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     2401 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     2461 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     2521 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     2581 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     2641 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     2701 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     2761 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     2821 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     2881 tcattttacg tttctcgttc agctttcttg tacaaagtgg tgataaccta gagctcgaat
     2941 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     3001 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     3061 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3121 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3181 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     3361 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     3421 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     3481 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     3541 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     3601 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgcctc gagtgacttc
     3661 tctgattgca gactatttat accaaccagc aagaaagtgt cgaagtatga agcagaaggc
     3721 gcaggctaga gcctggaaaa cgagaagatg gatgcaaaga gacttttctg atttaggacc
     3781 aaacaacctc aagtaaacat tgacgagtgt cccatctttt gtgccggtca gtaatccggt
     3841 agcaggatca taccttggga gacgatggcg cggacatttc acaatcccag caagctgagg
     3901 aagcgataat agaaggttca gaacttgagg aagaaagaag atgaggaggg tttcactgaa
     3961 gtgacccaga ataccaacaa ctgccatagt cattccggcg aagactgtgt aagtgtctcc
     4021 aacaaaaact gaagaaggat accaattgta agcaagcatt gccaaggatg tggccatcag
     4081 cggttgagta agaaatatcg agaaggcatg agcttggtga tactcgggat caacagatgc
     4141 tccgatttgc ataacgttgt gtatcaggat agcagcggca ataacaactg tttgaccgat
     4201 ttcaaggcca ttcagaccag cgtggatgtt aatagagttt gtacaaaaga cagcaagtag
     4261 ccccatatat aacttatata tccttcctag attaagcact tccaagccaa tataagcaac
     4321 aagaggtttt ggtataacaa tagttgtatg accagcataa gccatcaaaa gtggaagggt
     4381 agcaaaagat ggcaggacaa gttttactct ccaaggcaca tcaaggacat catctacaaa
     4441 tccaagcaaa atcatgaagc aaatagatgc tagtgctgca ttatactcca caagccaatt
     4501 agaatcttca gtgaagttaa aatactgaaa tattatcgcc acgatcaaaa agacaatacc
     4561 aacaacaata cccaatgact ctggcacttt aatatctcct tgaggcgtgc ctcttttgtt
     4621 gatatcaaaa ccaaacatgt tacgtctcag aacgtatctg gcagtaacag gaatcatttt
     4681 gagagtgacg aaaaacccaa ccaagctaag ccccgcgtta attagaatcg atcgcttgag
     4741 ttcattatca accttgtagt gaaaacagag aaggtataga tagagcgaac agagaagagt
     4801 agagatcacg aagatcactc ctaatttcgg cggcgcgaga cgaaattcct caccggaagc
     4861 agaaaccgtc tttcgggtca tcttctgctc cgatggcgct gagttgggtt ccgacgggtc
     4921 cggtttgttg ggaattgaga tcgatgaagc tctcttacga gctgccatat cttattgccc
     4981 cccgggatca gatctggatt gagagtgaat atgagactct aattggatac cgaggggaat
     5041 ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta
     5101 ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca
     5161 gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac
     5221 ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
     5281 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     5341 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     5401 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     5461 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     5521 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
     5581 gtgcttgcgg cagcgtgaag ctgatccgtc gagattaata gaaataaatt cagcctaatt
     5641 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     5701 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     5761 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     5821 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     5881 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     5941 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
     6001 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     6061 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     6121 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     6181 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     6241 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     6301 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     6361 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     6421 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     6481 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     6541 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     6601 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     6661 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     6721 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     6781 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     6841 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     6901 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     6961 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     7021 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     7081 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
     7141 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     7201 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7261 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7321 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7381 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7441 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7501 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7561 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7621 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7681 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7741 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7801 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7861 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7921 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7981 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8041 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     8101 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8161 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     8221 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8281 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     8341 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     8401 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     8461 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     8521 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     8581 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     8641 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     8701 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
     8761 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     8821 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     8881 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     8941 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     9001 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     9061 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     9121 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     9181 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     9241 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     9301 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     9361 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     9421 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     9481 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     9541 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     9601 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     9661 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
     9721 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     9781 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     9841 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
     9901 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
     9961 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    10021 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    10081 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    10141 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    10201 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    10261 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    10321 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    10381 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    10441 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    10501 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    10561 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    10621 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    10681 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    10741 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    10801 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    10861 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    10921 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    10981 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    11041 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    11101 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    11161 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    11221 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    11281 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    11341 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    11401 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    11461 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    11521 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    11581 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    11641 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    11701 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    11761 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    11821 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    11881 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    11941 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    12001 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    12061 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    12121 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    12181 cct
//
